摘要
本研究比较黑灵芝不同溶剂提取物的抗氧化活性。依次用石油醚、氯仿、甲醇和水提取黑灵芝,采用DPPH自由基法、Folin-Ciocalteu法和FRAP法分别研究其不同溶剂提取物的抗氧化活性。结果表明:黑灵芝的不同溶剂提取物均具有较强的抗氧化活性,且其抗氧化能力均呈一定的量效关系。同时,研究还表明,黑灵芝的不同溶剂提取物对DPPH自由基的清除能力之间存在着协同作用。结论:黑灵芝抗氧化活性物质主要是乙酸乙酯提取物,其次是水提取物。
Dried Ganoderma lucidum was sequentially extracted with petroleum ether, chloroform, methanol and water and the methanol extract was fractionated into ethyl acetate- and n-butyl alcohol-soluble fractions. The antioxidant abilities of the extracts from Ganoderma lucidum obtained were evaluated by DPPH radical scavenging, Folin-Ciocalteu and FRAP assays. All the extracts exhibited strong antioxidant abilities in a dose-dependent manner. These extracts from Ganoderma lucidum had a synergistic effect in the DPPH scavenging assay. The ethyl acetate-soluble fraction had the strongest antioxidant activity, followed by the water extract.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2009年第17期98-101,共4页
Food Science
基金
国家“863”计划项目(2008AA10Z325)
食品科学与技术国家重点实验室目标导向资助项目(SKLF-MB-200806)
江西省科技厅科技攻关重点项目
关键词
黑灵芝
抗氧化活性
协同作用
Ganoderma lucidum
antioxidant activity
synergistic effect