大豆7S球蛋白碱性蛋白酶酶解产物的结构表征

Structural Characterization of Alcalase Hydrolysates of Soybean 7S Globulin

7S和11S球蛋白为大豆蛋白主要组分,对大豆蛋白的物化特性有重要的作用。本研究利用SDS-聚丙烯酰胺凝胶电泳(SDS-PAGE)、扫描电镜(SEM)、傅立叶红外光谱(FTIR),分析大豆7S球蛋白及其不同水解度的碱性蛋白酶酶解产物的结构特征;通过疏水性和巯基含量的测定,分析水解度对7S球蛋白的疏水性和巯基含量的影响。实验结果表明:碱性蛋白酶酶解导致7S球蛋白的构象发生很大变化,而构象的变化取决于水解的程度。

Soybean 7S and 11S globulins, two major soybean protein components, play an important role in maintaining the phisico-chemical properties of soybean proteins. The structural properties of both soybean 7S globulin and its hydrolysates were investigated by SDS-PAGE, SEM and FTIR infrared spectroscopy, meanwhile their hydrophobicity and sulfhydryl content were measured. That alcalase-eatalyzed hydrolysis led to marked conformational changes was observed in soybean 7S globulin and the degree of change was dependant on the degree of hydrolysis.