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人caveolin-1基因慢病毒过表达载体构建及其对SMMC7721细胞凋亡的影响 被引量：2

Construction of Recombinant Lentivirol Overexpression Vector Carring Human Caveolin-1 and its Correlation with Apoptosis of SMMC7721 Cell Line
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摘要 目的构建人caveolin-1基因慢病毒过表达载体,并研究其对人肝细胞癌株SMMC7721细胞凋亡的影响。方法应用慢病毒载体系统构建人caveolin-1基因pGC-FU-caveolin-1过表达质粒,并转导SMMC7721细胞,检测其转导效率、细胞内caveolin-1 mRNA和蛋白水平的改变和对细胞凋亡的影响。结果pGC-FU-caveolin-1过表达质粒慢病毒转导SMMC7721细胞72 h的转导效率为(96.3±1.6)%。转导72 h和120 h后caveolin-1 mRNA水平分别是阴性慢病毒感染组的(30.15±1.22)倍和(189.16±28.13)倍,两个时相点的差别均具有统计学意义(P<0.05)。同期转导后120 h的蛋白水平检测明显升高。pGC-FU-caveolin-1过表达质粒慢病毒转导SMMC7721细胞120 h后能使早期细胞凋亡数减少约70%,两者差别具有统计学意义(P<0.05)。结论成功构建的pGC-FU-caveolin-1过表达质粒慢病毒能在SMMC7721细胞中长期稳定表达,其过表达能显著抑制SMMC7721细胞凋亡。 Objective To construct the lentiviral overexpression vector of humon caveolin-1 and investigate its correlation with apoptosis of SMMC7721 cell line. Methods SMMC7721 cells were infected with recombinant lentivirus overexpressing human caveolin-1,and the transduction efficiency,the caveolin-1 mRNA and protein levels and the effects of caveolin-1 overexpression on the induced apoptosis of SMMC7721 cells were investigated. Results The transduction efficiency of SMMC7721 cell line with pGC-FU-caveolin-1 lentivirus was （96.3± 1.6）%. 72 h and 120 h after virus infection, the caveolin-1 mR-NA levels were （30.15±1.22） times and （189.16±28.13） times more than negative control group, respectively （P〈0.05）. The caveolin-1 protein level was significantly increased. 120 h after infection, the amount of induced apoptotic SMMC7721 cells was significantly decreased about 70% （P〈0. 05）. Conclusion Human caveolin-1 can be stably overexpressed in SMMC7721 cells by recombinant lentivirus infection, which leads to a significant inhibition of induced apoptosis in those cells .
作者 李国平 潘润阳 张志波 何庆良 石铮
机构地区 福建医科大学附属第一医院病理科 福建医科大学附属第一医院肝胆胰外科
出处 《福建医科大学学报》 2009年第4期297-300,共4页 Journal of Fujian Medical University
基金 福建省自然科学基金资助项目(2008J0083)
关键词 细胞质膜微襄蛋白 癌 肝细胞 慢病毒属 遗传载体 细胞凋亡 肿瘤细胞 培养的 caveolins carcinoma hepatocelluar lentivirus genetic vectors apoptois tumor cells cultured
分类号 R735.7 [医药卫生—肿瘤] R394.3 [医药卫生—医学遗传学]
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参考文献8

  • 1张志波,蔡磊,郑树国,熊燕,董家鸿.Caveolin-1 RNA干扰慢病毒载体的构建及其对肝癌细胞株SMMC7721侵袭的影响[J].第三军医大学学报,2009,31(10):931-934. 被引量：2
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二级参考文献8

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  • 8Rubinson D A, Dillon C P, Kwiatkowski A V, et al. A lentivirusbased system to functionally silence genes in primary mammalian cells, stem cells and transgenic mice by RNA interference [ J ]. Nat Genet, 2003, 33 (3) : 401 -406.

共引文献1

同被引文献20

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引证文献2

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福建医科大学学报
2009年 第4期

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