hsa-miR-125a-5p促进非小细胞肺癌细胞侵袭

hsa-miR-125a-5p Enhances Invasion Ability in Non-Small Lung Carcinoma Cell Lines

背景与目的microRNAs(miRNAs)是小的非编码RNA,它们通过与靶mRNA不完全互补连接的方式转录后调控靶基因的表达。尽管一些失常的miRNA调节机制在人许多恶性肿瘤中被发现,但是,hsa-miR-125a-5p在肺癌细胞中的表达及功能还尚不清楚。本研究探讨了hsa-miR-125a-5p在非小细胞肺癌细胞中的表达及其与肺癌细胞侵袭的关系。方法应用real-timePCR的方法检测了hsa-miR-125a-5p在6种肺癌细胞系中的表达情况,并在A549细胞中分别转染正义的hsa-miR-125a-5p2’-O-甲基寡核苷酸24h、36h、48h、60h和72h后检测hsa-miR-125a-5p的表达情况。同时应用transwell小室观察上调hsa-miR-125a-5p后,A549细胞侵袭能力的变化。结果hsa-miR-125a-5p在6种肺癌细胞系中低表达,以LH7、NCI-H460、SPC-A-1和A549细胞最为明显。在A549细胞中,转染正义的hsa-miR-125a-5p2’-O-甲基寡核苷酸36h后,hsa-miR-125a-5p表达最高,并进一步发现,上调hsa-miR-125a-5p后A549和NCI-H460细胞侵袭能力增强。结论hsa-miR-125a-5p在肺癌细胞中低表达,但上调hsa-miR-125a-5p能够促进A549和NCI-H460细胞侵袭。

Background and objective MicroRNAs （miRNAs） are short non-coding RNAs that posttranscriptionally regulate gene expression by partially binding complementary to target sites in mRNAs. Although some impaired miRNA regulations have been observed in many human cancers, the functions of miR-125a are still unclear. The aim of this study is to investigate the expression ofhsa-miR-125a-Sp in NSCLC cell lines and the relationship between hsa-miR-125a-Sp and the invasion of lung cancer cells. Methods The expression of hsa-miR-125a-Sp and the effectiveness for a given period time after being transfected sense hsa-miR-125a-Sp 2＇-O-methyl oligonucleotide, which were 24 h, 36 h, 48 h, 60 h and 72 h, were examined by real- time PCR. Meanwhile, we investigated the modification ofinvasive ability in A549 and NCI-H460 ceils by transwell. Results Real-time PCR showed that hsa-miR-125a-Sp was poorly-expressed in 6 lung cancer cell lines, especially in LH7, NCI-H460, SPC-A-1 and A549. The highest expression of hsa-miR-125a-Sp occurred in the cells transfected with sense hsa-miR-125a-Sp 2＇-O-methyl oligonucleotide 36 h. Furthermore, the invasive abilities ofA.549 and NCI-H460 were enhanced by up-regulating hsa-miR-125a-Sp. Conclusion hsa-miR-125a-Sp was poorly-expressed in lung cancer cells and it could enhance lung cancer cell invasion by up-regulating hsa-miR- 125a-Sp.