三种缝线材料对人肺腺癌细胞A549增殖和细胞周期的影响

Effect of Suture Lines on Lung Adenocarcinoma Cell in vitro

背景与目的细胞与生物医用材料之间的相互作用是影响其临床应用的重要因素之一,本研究旨在探讨三种缝线材料对人肺腺癌细胞A549增殖和细胞周期的影响。方法将三种缝线材料分别与A549细胞直接培养72h后,采用MTT法测定各组吸光度,反映各组A549细胞的增殖情况,用流式细胞技术检测各组G1期细胞和S期细胞所占比例。结果不同缝线材料对A549细胞增殖和细胞周期的影响不同(P<0.05),可吸收线对A549细胞增殖的影响最强,丝线次之,滑线最弱;同一缝线材料不同长度对A549细胞增殖和细胞周期的影响不同(P<0.05)。结论三种不同缝线材料对人肺腺癌细胞A549的增殖和细胞周期的影响不同,且呈现剂量效应关系。

Background and objective The interaction of cell and medical biomaterial is one of the signif icant factors to affect clinical application of medical biomaterial.This research is to investigate three of suture lines how to affect the proliferation and cell cycle of Lung Adenocarcinoma cell A549.Methods Three of suture lines are respectively cultivated with Lung Adenocarcinoma cell A549,after of 72 hours,to detect absorptance of each groups by MTT method in order to reflect the proliferation of Lung Adenocarcinoma cell A549 and to detect percentage of G1 period cell and S period cell of each of groups by flow cytometry.Results Different of suture lines have effect differently on the proliferation and cell cycle of Lung Adenocarcinoma cell A549(P<0.05).The effect of absorbent suture line on the proliferation and cell cycle of Lung Adenocarcinoma cell A549 is strong,the effect of chorda serica chirurgicalis is medium,the effect of slide wire is poor.Different length of each suture line have effect differently on the proliferation and cell cycle of Lung Adenocarcinoma cell A549(P<0.05).Conclusion Three of suture line materials have different effect on the proliferation and cell cycle of Lung Adenocarcinoma cell A549,to reflect dose-effect relation.