溶胶-凝胶法制备的二氧化硅中肌红蛋白的苯胺羟化酶活性

ANILINE HYDROXYLASE ACTIVITY OF MYOGLOBIN ENCAPSULATED IN SILICATE SOL GELS

用溶胶－凝胶制备了多孔透明的二氧化硅包埋肌红蛋白，在还原辅酶Ⅰ二钠盐（ＮＡＤＨ）和黄素单核苷酸（ＦＭＮ）的存在下，用可见光谱法测定了肌红蛋白苯胺羟基化反应的Ｋｍ和ｋｃａｔ。表明包埋在二氧化硅中的肌红蛋白保持了苯胺羟化酶活性。

Using sol gel procedures, we encapsulated myoglobin in the porous and transparent silicate glass The transparency of sol gel makes it feasible for us to monitor the hydroxylation reaction spectroscopically in the pore of the matrix Thus the K m and k cat values of the hydroxylation reaction for Mb sol gel were determined to be 10 3 mmol·L -1 and 0 013 min -1 , respectively It shows that the aniline hydroxylase activity of myoglobin (Mb) encapsulated in silicte sol gels in maintained and can be induced by a semi artificial multienzyme system of NADH FMN Mb