硅胶聚合物键合相键合基团及流动相盐浓度和pH值对蛋白质分离的影响

Preparation of Silicage-Polymer - Bonded Phase and the Effect of Different Agent, pH and Salt Concentration in Elution on Proteins Separation

介绍了３种同孔径不同键合基团的大孔径硅胶聚合物键合相（ＨＤＳＩ，ＰＡＭＳＩ，ＰＦＭＳＩ）的合成，３种填料是在 １００ ｎｍ孔径硅胶上键合乙烯基后，分别与甲基丙烯酸羟乙基酯、二乙烯基苯共聚形成ＨＤＳＩ填料；与甲基丙烯酸胺共聚形成ＰＡＭＳＩ填料；与顺丁烯二酸共聚再与乙二胺反应形成ＰＦＭＳＩ填料。比较了它们对蛋白质分离的特性。结果表明：３种填料都能达到蛋白质快速分离。并且讨论了在ＰＡＭＳＩ色谱柱上，盐浓度对蛋白质分离的影响；在ＰＦＭＳＩ色谱柱上。

The synthesis of three (100 nm) macroporous silicage-polymer- bonded packings (HDSI, PAMSI, PFMSI) and their chromatographic characteristics in the separation of proteins are introduced. The results show that macroporous packings all can separate proteins quickly. The effect of salt concentration in elution on the retention of proteins on PAMSI packing is discussed. The effect of pH in mobile phase on the retention of proteins on PFMSI packing is also discussed.