乳腺浸润性导管癌患者血清HIF-1α和Gly-G含量的变化及其意义

Changes of serum HIF-1α and Gly-G in patients with invasive ductal breast cancer

目的探讨乳腺浸润性导管癌血清低氧诱导因子-1α(HIF-1α)和甘氨酸G(Gly-G)的含量变化及其临床意义。方法采用ELISA方法检测117例乳腺原发浸润性导管癌(A组)、10例复发浸润性导管癌(B组)、20例乳腺良性肿瘤(C组)和31例正常人(D组)血清HIF-1α,采用高效液相色谱法分析血清Gly-G的含量及其在不同病理特征中的变化。结果A组血清中HIF-1α水平明显高于C、D组,B组HIF-1α高于A组;A、B组血清Gly-G含量低于C、D组。血清HIF-1α和Gly-G水平与乳腺浸润性导管癌病理分期、组织学分级、肿瘤大小、淋巴结转移密切相关;HIF-1α与Gly-G呈负相关。结论乳腺浸润性导管癌血清HIF-1α和Gly-G含量的异常变化可能是癌变过程的进展行为,而血清中HIF-1α和Gly-G的水平可能成为评价乳腺浸润性导管癌病理分期、组织学分级、肿瘤大小、淋巴结转移等生物学重要指标。

Objective To study the changes of serum hypoxia-inducible factor-1α（HIF-1α） and Oly-G in patients with invasive ductal breast cancer. Methods Serum HIF-1α was detected with ELISA in 117 patients with breast infiltration catheter cancer（group A）, 10 cases of recrudesced patients（group B）,20 cases with breast benign turnouts（group C） and 31 healthy persons（group D）. The changes of serum Gly-G were examined with HPLC and the relationship between the changes of Gly-G and different pathological features was analyzed. Results Serum levels of HIF-1α and Gly-G were closely correlated with the size, clinical stage, pathological and histological classification and lymph metastasis of primary invasive ductal breast cancer. Serum HIF-1α was negatively correlated to Gly-G. Conclusion The abnormal changes of serum HIF-1α and GlyG are related to the progression of invasive ductal breast cancer, which may be the markers for evaluating its size, pathological stage and histological classification and lymph node metastasis.