类风湿关节炎患者CD4^+T细胞亚群4-1BB和CD28分子的表达及临床意义

Expression and significance of serum 4-1BB and CD28 on CD4^+T lymphocyte subsets in patients with rheumatoid arthritis

目的探讨类风湿性关节炎(RA)患者CD4+T细胞4-1BB和CD28的表达及其与疾病的关系。方法根据DAS积分将RA组分为活动期组和稳定期组,用多色荧光流式细胞技术检测CD28、4-1BB在RA组和正常对照组外周血T细胞上的表达。结果与对照组比较,RA组CD4+T细胞增高,CD8+T细胞降低,CD4+CD28-T细胞频数增高(P<0.05)。RA组4-1BB在CD4+T细胞和CD4+CD28-T细胞上的表达明显高于对照组(P<0.05)。活动期组CD4+CD28-T细胞频数和4-1BB在CD4+T细胞上的表达高于稳定期组(P<0.05)。结论共刺激分子CD28在RA患者自身免疫性T细胞中表达减少,而4-1BB表达相应增高,这可能与此类自身免疫反应细胞组织损伤活性增强。

Objective To study the expression and significance of serum costimulatory molucules on CD4^＋ T lymphocyte subsets in patients with rheumatoid arthritis （RA）. Methods Peripheral blood T cell subsets and the expressions of 4-1BB and CD28 on T cells in RA patients Eactive（group A） and inactive（group Ⅰ）5 and normal controls（group C） were detected with multicolor flow cytometry. Results The CD4^＋ T cell was higher, CD8^＋ T cell was lower and the frequency of CD4^＋ TCD28^- T cells was higher in RA patients than those in group C （P〈0.05）. The frequencies of CD4^＋CD28^-T cells and expression of 4-1BB on CD4^＋ cells were higher in group A than those in group Ⅰ （P〈0.05）. Conclusion The expression of CD28 on autoimmune T ceils is decreased and that of 4-1BB is incresed in RA patients, which may be related to the augmentation of maladaptive immune responses.