醇类改性剂对胶束电动色谱分离的影响

Effect of Alcohol Modifier on Micellar Electrokinetic Chromatography Separation

胶束电动色谱（ＭＥＫＣ）是毛细管电泳常用的一种分离模式，它能使电中性化合物得到高效的分离．ＭＥＫＣ的分离度可通过调节电解质溶液的组成来提高．其中，有机改性剂的使用是近几年来ＭＥＫＣ研究的重点之一［１，２］．已有文献［３～５］报道以醇类作为电解质溶液的...

The effect of the addition of normal C1—C5 alcohols on the background electrolyte on the separation of a set of wide polar aromatic hydrocarbons in micellar electrokinetic capillary chromatography(MECC) was examined. Experiments were carried out with the micellar solution system of sodium dodecyl sulfate borate at pH 9 2, containing different amount(0—15%) of C1—C5 alcohols separately. For the shorter alkyl chain C1—C3 alcohols, as the content of alcohol increased, the elution window( t m/t 0) increased, and the capacity factors( k ) of all test samples decreased. Comparatively, C4 and C5 alcohols had the different effects. With the content increased the t m/ t 0 increased first and then decreased, and the k  increased for the most solutes. The results also showed that the alcohol additives had more effect on weakly polar or hydrophobic compounds than more polar compounds. The addition of alcohol caused the increase of both the number of theoritical plates( N ) and the resolution, especially for the hydrophobic compounds.