蝴蝶兰组织培养快速繁殖技术研究

Research on Tissue Culture and Rapid Propagation of Phalaenopsis amabilis

[目的]对蝴蝶兰的组织培养快速繁殖技术进行研究,为蝴蝶兰的批量生产提供参考。[方法]以蝴蝶兰嫩叶、茎尖、花梗侧芽为外植体,进行蝴蝶兰原球茎诱导、增殖及试管苗生根的组织培养。[结果]结果表明,MS+2.0mg/L6-BA+1.0mg/LNAA+200ml/L椰乳是诱导蝴蝶兰原球茎形成的最佳培养基,茎尖诱导率达81.37%;MS+1.0mg/LNAA+2.0mg/L6-BA是蝴蝶兰原球茎增殖的最佳培养基,增殖系数达9.62;1/2MS是蝴蝶兰生根培养的最佳培养基,生根率达94.42%,且根生长最快最整齐。[结论]在生产上,可采用试管苗生根继代培养的方法进行蝴蝶兰的快速繁殖。

[ Objective ] The aim was to research the tissue culture and rapid propagation of Phalaenopsis amabilis, and provide reference for batch production of Phalaenopsis amabilis. [ Method] Taking tender leaves, the shoot -tips and pedicel lateral buds as explants, the conditions of original corm induction, proliferation and rooting of Phalaenopsis amabilis were discussed. [ Result] The result showed that the optimum medium for inducing the tender protocorm of Phalaenopsis amabilis was MS ＋ 2.0 mg/L 6-BA ＋ 1.0 mg/L NAA ＋ 200 mL/L coconut milk, and the inducement rate of shoot-tip was 81.37%. The optimum medium for protocorm proliferation of Phalaenopsiz amabilis was MS ＋ 1.0 mg/L NAA ＋ 2.0 mg/L 6-BA, and the proliferation coefficient was 9.62. The optimum medium for rooting of Phalaenopsis amabilis was MS, the rooting rate was 94.42% and the root growth was fastest and most regular. [ Conclusion] In the production, the rapid propagation of Phalaenopsis amabilis should be made by using the rooting subculture method of test-tube seedlings.