乙基紫-脱氧核糖核酸共振发光体系的研究及其分析应用

Studies on the Resonant Luminescence Spectra of Ethyl Violet with Deoxyribonucleic Acid and Its Analytical Application

研究了碱性染料乙基紫与小牛胸腺脱氧核糖核酸（ｃｔＤＮＡ）体系的结合反应及其共振发光光谱．在ｐＨ５．０～１０．０范围内，乙基紫在５９５ｎｍ处有一特征吸收峰，加入ＤＮＡ后，吸收峰消失，而在５０７ｎｍ处出现新的吸收峰，表明有新的结合物形成．在５１０ｎｍ处出现共振发光峰，其发光强度与ＤＮＡ浓度呈线性关系，线性范围为０～５００ｎｇ／ｍＬ；检出限（３σ）为１．５４ｎｇ／ｍＬ．方法具有较高的灵敏度和准确度．将该体系用于核酸试样的测定。

The binding reaction and resonant luminescence spectra of ethyl violet with ctDNA have been studied. In the pH range of 5 0—10 0 ethyl violet exhibited a maximum of absorption at 595 nm. A new absorption peak at 507 nm appeared when ctDNA was added. It showed that a binding product of ethyl violet with DNA formed. The product emitted a sharp resonant luminescence peak at 510 nm. The intensity of resonant luminescence was found to be dependent on the concentration of DNA. The linear range of the calibration graph was 0—500 ng/mL. The limit of detection(3 σ ) was 1 54 ng/mL. A new method of resonant luminescence for the determination of trace DNA was established with high sensitivity and selectivity.