猪繁殖与呼吸障碍综合征的诊断及防治

Diagnosis and Prevention of Porcine Reproductive and Respiratory Syndrome(PRRS)

[目的]减轻猪繁殖与呼吸障碍综合征对养猪业的危害。[方法]对某猪场病死猪进行病理剖检,采集病猪肺脏、脾脏和淋巴等组织,经过研磨、冻融、加双抗、离心后取上清液。[结果]采用RT-PCR方法进行实验室检测,结合病死症状及实验室结果,初步确定为感染PRRSV;根据室验室结果,采取相应的防治措施,已取得了较理想的效果。[结论]该研究为今后猪感染高致病蓝耳病的诊断及防治提供了重要依据。

[ Objective ] The research aimed to relieve the harm of blue-eared pig disease to swine industry. [ Method ] Pathologic autopsy of swine died from illness was carried out. After collecting lung, spleen, lymph tissue, etc. from body of swine, we run grind, freeze thawing, adding double-antibody, and centrifugalization, then aspirated superuate. [ Result ] With the detection of RT-PCR, combining the symptom of death, PRRSV infection was determined preliminarily. According to the experiment results,prevention measures were adopted and correspondingly fairly ideal effect was obtained. [ Conclusion] The research provided important basis for diagnosis and prevention of highly pathogenic porcine reproductive and respiratory syndrome （PRRS）.