蜡蚧轮枝菌MZ041024菌株对平菇厉眼蕈蚊成虫的毒力测定

Determination on the Virulence of Verticillium lecanii Strain MZ041024 to Adult Lycoriella pleuroti

[目的]研究蜡蚧轮枝菌MZ041024菌株对平菇厉眼蕈蚊成虫的致病力。[方法]以采集于蒙自食用菌栽培地的菌袋上的平菇厉眼蕈蚊为试材,蜡蚧轮枝菌MZ041024菌株的分生孢子浓度共设5.0×104～5.0×108个/ml的5个处理浓度,对平菇厉眼蕈蚊成虫进行室内毒力测定。[结果]蜡蚧轮枝菌MZ041024菌株对平菇厉眼蕈蚊成虫的毒力随分生孢子浓度的增加而增强,第2天开始出现平菇厉眼蕈蚊成虫死亡,在5.0×104～5.0×108个/ml的5个浓度处理的成虫死亡率分别为31.92%、45.16%、62.25%、73.03%和79.78%;第4～7天的致死中浓度(LC50)分别为3.581×108、2.451×107、4.218×106和2.648×105个/ml;5.0×105～5.0×108个/ml浓度下成虫的致死中时间(LT50)随着分生孢子浓度的增加而逐渐缩短,由7.5d缩短到4.8d。[结论]蜡蚧轮枝菌MZ041024菌株在室内对平菇厉眼蕈蚊成虫具有较强的毒力和较好的防治效果。

[ Objective ] The purpose was to study the pathogenicity of Verticillium lecanii MZ041024 to the adult of LycorieUa pleuroti. [ Method ] With L. pleuroti selected from the fungi bags in the edible fungi cultivation land in Mengzi County as the tested material, the conidium concn, of V. lecanii MZ041024 was set up as 5 treating concn, from 5.0×10^4～5.0×10^8 spores/ml to make the determination on the virulence to the adult of L. pleuroti in lab. [ Result] The virulence of V. lecanii to the adult ofL. pleuroti was strengthened along with the increase of conidium concn, and the adult of L. pleuroti appeared to be dead from 2nd d, with the mortality rate of adult was 31.92%, 45.16%, 62. 25% , 73.03% and 79.78% resp. under 5 conidium concn, from 5.0×10^4～5.0×10^8 spores/ml. LC50 was 3.581×10^8、2.451×10^7、4.218×10^6 and 2.648×10^5 spores/m] in 4^th, 5^th, 6^th, 7^th d resp. LT50 of adult under 5 conidium concn, from 5.0×10^5～5.0×10^8 spores/ml was shortened along with the increase of conidium concn, and it was shortened from 7.5 d to 4.8 d. [ Conclusion ] V. lecanii MZ041024 had stronger virulence and better control effect on the adult of L. pleuroti in the lab.