胎儿微嵌合体对Graves病动物模型甲状腺内树突状细胞的影响

The influence of fetal microchemisrism to dendritic cells in the thyroid glangs

目的:分析Graves病动物模型甲状腺内胎儿微嵌合体和树突状细胞浸润和成熟程度间的关系,探讨胎儿微嵌合体影响Graves病发病的可能机制.方法:利用Ad-TSHR289制备Graves病动物模型进行配对,Real-time PCR测定妊娠前后甲状腺内Y染色体特异性基因序列SRY,免疫组化染色各组甲状腺组织内树突状细胞的特异性标记S-100和成熟树突状细胞的特异性标记CD80.结果:免疫生育组和免疫妊娠组甲状腺内树突状细胞多于单纯免疫组,成熟树突状细胞在免疫生育组最多(P<0.05).免疫生育组中SRY基因相对表达量与树突状细胞浸润程度成正相关(r=0.5648,P<0.05).无论是在免疫妊娠组还是在免疫生育组,SRY基因相对表达量与成熟树突状细胞浸润程度均为正相关关系(r=0.4262,P<0.05).结论:甲状腺内胎儿微嵌合体可能通过促进树突状细胞成熟来改变甲状腺局部的免疫状态,从而促进产后Graves病的发生和加重.

AIM： To analyze the relationship between fetal microchemisrism and dendritic cells （ DCs ） in the thyroid tissue to explain the influence of the fetal microchemirism to Graves＇ disease（GD）. METHODS： Saventy-two mice were injected with Ad-TSH289 （5 × 10^8 IU ） and mate with male mice until 2 weeks of pregnancy or 2 weeks post-partum. SRY gone was tested by re-al-time PCR. S-100 protein and CD80 were used as the special marker of the DCs and mature DCs respectively. These markers were stained in the thyroid tissue of GD animal model by HC. RESULTS ： The number of the S-100 positive cells in the thyroid tissue of pregnancy and post-partum GD animal model group was more than that of the simple immunized GD animal models （ P 〈 0.05 ）. But the number of CD80 positive cells of post-partum group was the most among those 3 groups （ P 〈 0.05 ）. There were statistically positive co-relationship between relative expression level of SRY gene and number of dendritic cells （ S-100 positive cells） in the postpartum GD models（ r = 0.5648, P 〈 0.05 ）. At the same time, there were statistically positive co-relationship between level of SRY gene and mature dendritic cells （ CD80 positive cells ） in the pregnancy and post-partum GD models （r = 0.4262, P 〈 0.05 ）. CONCLUSION ： Intrathyroidal fetal micro- chimerism may play a very important role to modulate the autoimmune response for post-partum GD animal models by influencing the mature of dendritic cells in the thyroid glands.