调衡方对Lewis肺癌荷瘤小鼠免疫功能调节的实验研究

Tumor inhibiting and immunoloregulation effects of Tiaoheng Prescription on Lewis lung carcinoma of tumor-bearing mice

目的:研究调衡方对小鼠Lewis肺癌自发性肺转移的作用并探讨其作用机制.方法:将传代培养的Lewis肺癌细胞接种于C57BL小鼠右腋皮下建立Lewis肺癌模型;灌胃给药12 d后观察瘤组织中淋巴细胞与瘤细胞形态学的变化;常规石蜡切片HE染色.观察瘤组织癌细胞形态的变化;免疫组化法检测瘤组织中CD4+,CD8+的表达水平及其血清IL-2的活性.结果:调衡中、大剂量组可见瘤组织Lewis肺癌细胞大量坏死和凋亡,荷瘤对照组对肿瘤细胞形态无明显影响;调衡中剂量组和调衡大剂量组瘤组织中CD4+与CD8+表达均增多,而阳性组、调衡小剂组、热休克组则主要是CD8+表达升高.调衡方在25 g/kg剂量下,对Lewis肺癌荷瘤小鼠外周血IL-2活性水平有显著地增强作用.结论:调衡方对Lewis肺癌荷瘤小鼠的抑瘤作用机制可能与增强小鼠外周血IL-2的活性有关及增强CD4+,CD8+的表达水平,从而阻止了肿瘤细胞的侵袭转移.

AIM： To investigate the mechanisms of tumor inhibiting and immunoloregulation of ＂Tiaoheng Prescription＂ on Lewis lung carcinoma of Tumor-bearing Mice. METHODS ： The mouse model of Lewis lung carcinoma of tumor-bearing mice was established and then the mice were given Tiaoheng Formula by intragastric administration. Twelve days later, the mice were executed and the tumour tissue smears were prepared. The cell feature in tumour tissue was observed under light microscope, and the expression of CD4 ＋ and CD8 ＋ on the cellular membrane of the T lymphocyte in tumour tissue and the level of IL-2 in the peripheral blood was detected with immunohistochemical or ELISA method. RESULTS ： A large amount of damaged and apoptotic Lewis lung carcinoma cells were found in Tiaoheng Prescription midst and large dosage group, and the changes of cell feature in control group were not obvious . The expressions of CD4 ＋ and CD8 ＋ on the membrane of T cells in tumour tissue were increased in Tiaoheng Prescription midst and large dosage groups, and CD8 ＋ expression was increased in heat-shock group, masculine group, and Tiaoheng Prescription minidose groups. The production of IL-2 by immunocyte obviously enhanced in Tiaoheng Prescription [25 g/（kg · d） by gavage for 12 d]- treated group（P 〈0.01）. CONCLUSION： Tiaoheng Prescription can inhibit the cancer cell of Lewis lung carcinoma in mice. The mechanism may be related to that Tiaoheng Prescription Can enhancement of T lym- phocyte immunological function by regulating the expression of T lymphocyte CD4 ＋ and CD8 ＋ molecules in Lewis lung carciuoma, enhance the activity level of IL-2 in the peripheral blood, and to prohibit the invasion and metastasis of tumor cells.