大鼠体内有机阴离子转运多肽2表达的年龄特征

Age characteristics of Oatp2 in rat

目的:研究正常大鼠脑、肝内有机阴离子转运多肽2(Oatp2)表达的年龄特征.方法:选择健康成年、生后20 d(P20)和3 d(P3)的Wistar大鼠各12只,雌雄各半.取脑、肝组织,免疫组化及RT-PCR分别检测Oatp2蛋白及mRNA表达,比较年龄因素对Oatp2表达的影响.结果:大鼠肝组织内Oatp2蛋白表达以P3组最低(234.596±52.529),P20组次之(244.256±68.547),成年组表达最高(257.449±56.323);脑内Oatp2表达以P3组最高(280.434±52.331),P20组次之(264.646±59.050),成年组最低(261.806±57.465).RT-PCR检测发现肝、脑内Oatp2-mRNA表达与蛋白表达变化规律基本相同.结论:正常大鼠脑、肝内Oatp2表达与年龄因素相关,大鼠脑内Oatp2表达呈随年龄增加表达下降趋势,而肝内Oatp2表达则随年龄增加而增加.

AIM： To explore whether the expression of Oatp2 in rat brain and liver is different among rats of different ages. METHODS：Wistar rats were divided into 3 groups according to age： adult group, infant group （20 d after birth）and newborn group（3 d after birth） ,there were 12 rats in each group, and the numbers of male and female rats were equal. Expression of Oatp2 protein and mRNA were detected by immunohistochemistry and RT-PCR respectively. RESULTS：The Oatp2 expression quantity is different in different age and different tissue. In brain, newborn group（280. 434 ± 52. 331 ） express Oatp2 most, the infant group （264. 646 ± 59. 050 ） express Oatp2 more and the adult group （261. 806 ± 57. 465 ） express Oatp2 protein least. Whereas, expression of Oatp2 in liver gradually increased after birth, that is, the expression quantity of adult group （ 257. 449 ± 56. 323 ） is the highest, the infant group （ 244. 256 ± 68. 547 ） take second place and the newborn group （ 234. 596 ± 52. 529 ） is the least. CONCLUSION ： The age-denpendent feature of Oatp2 in rat bran and liver will aid in understanding the pharmacokinetics and toxieokinetics of drugs and other chemicals.