三甲益肝颗粒含药血清对HSC-T6细胞TGF-β1 mRNA和TIMP-1 mRNA表达的影响

Effect of Sanjiayi Gan granula contained serum on TGF-β1,TIMP-1 gene expression of HSC-T6 cells

目的:观察三甲益肝颗粒(S-JG)含药血清对肝星状细胞HSC-T6细胞转化生长因子β1(TGF-β1)mRNA和基质金属蛋白酶组织抑制因子1(TIMP-1)mRNA表达的影响.方法:不同的剂量的三甲益肝颗粒(1.7,3.4和6.8 g/kg)灌胃大鼠,制备含药血清,作用于HSC-T6细胞48 h,以逆转录定量PCR方法测定其对HSC-T6细胞TGF-β1 mRNA和TIMP-1mRNA表达的影响.结果:水飞蓟宾组,三甲益肝颗粒低、中、高剂量组的TGF-β1 mRNA,TIMP-1 mRNA表达水平分别为1.65±0.05,1.67±0.04,1.23±0.07,0.72±0.02,2.23±0.12,2.17±0.12,1.41±0.03,0.49±0.09,与对照组的TGF-β1 mRNA和TIMP-1 mRNA表达水平(2.11±0.11,2.72±0.11)比较,具有显著性差异(P<0.05).结论:三甲益肝颗粒降低HSC-T6细胞TGF-β1 mRNA,TIMP-1 mRNA表达水平,从分子水平上证明其抗肝纤维化的作用.

AIM： To observe the in vitro effect of Sanjiayi Gan granula （S-JG） contained serum on TGF-β1 mRNA, TIMP-1 mRNA expression of HSC-T6 cells. METHODS： HSC-T6 cells were exposed in different doses of Sanjiayi gan granula contained serum for 48 h. TGF-β1 mRNA, TIMP-1 mRNA levels were measured by quantitative reverse-transcription polymerase chain reaction （RT-PCR）. RESULTS： TGF-β1 mRNA, TIMP-1 mRNA levels of HSC-T6 cells at different doses of S-JG contained serum were 1.65 ± 0.05,1.67 ± 0.04,1.23 ± 0.07,0.72 ± 0.02 and 2.23 ± 0. 12,2.17 ±0. 12,1.41 ±0.03,0.49 ± 0.09,respectively, which were lower than those of normal control （2. 11 ± 0.11,2.72 ±0.11, P 〈0.05, respectively）. CONCLUSION： Sanjiayi gan granula （S-JG） contained serum can decrease TGF- β1 mRNA,TIMP-1 mRNA expression of HSC-T6 cells.