摘要
目的:制备IL-18基因修饰的树突状细胞AFP瘤苗,探索增强肝癌树突状细胞免疫治疗效果的新途径.方法:构建和制备rAd-IL18-AFP双基因表达的重组腺病毒,转染DC,应用RT-PCR和Western Blot方法检测IL-18和AFP基因在DC中的蛋白表达.结果:成功制备IL-18基因修饰的树突状细胞AFP瘤苗,rAd-IL18-AFP介导的IL-18和AFP基因能够在DC中进行蛋白表达.结论:成功制备IL-18基因修饰的树突状细胞AFP瘤苗,为进一步的肝癌免疫基因治疗研究打下基础.
AIM： To construct the IL18-AFP-DC vaccine mediated by IL-18 and AFP gene coexpressing adenovirns and to explore a new method of improving the immune response against hepatocellular carcinoma. METHODS： Cytokine interleukin-18 and AFP gene were transfected into dendritic cells （DC） mediated by adenovirus and the expressions of cytokine interleukin-18 and AFP gene in DC were detected by RT-PCR and Western blot. RESULTS： The IL18-DC-AFP vaccine was successful constructed. CONCLUSION： The IL18-AFP-DC vaccine mediated by IL-18 and AFP gene coexpressing adenovirus is successfully constructed, which has laid the basis for the downstream study of improving immune response against hepatocellular carcinoma.
出处
《第四军医大学学报》
CAS
北大核心
2009年第18期1768-1771,共4页
Journal of the Fourth Military Medical University
基金
福建省自然科学基金(C0540008)
福建医科大学附属协和医院博士科研启动基金(院管基金)
关键词
白细胞介素18
树突状细胞瘤苗
甲胎蛋白
肝癌
基因治疗
interleukin 18
dendritic cell vaccine
alpha-fetoprotein
hepatocellular carcinoma
gene therapy
