rhIGF-Ⅰ微球对人牙周膜成纤维细胞作用的实验研究被引量：1

Experimental study of the effects of rhIGF-Ⅰ-GMs on human periodontal ligament cells

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摘要目的:探讨重组人胰岛素样生长因子-Ⅰ明胶微球(recombinant human insulin-like growth factor-Ⅰ-gelatin microspheres,rhIGF-Ⅰ-GMs)保存rhIGF-Ⅰ生物活性的情况及其对人牙周膜成纤维细胞(human periodontal ligament fibroblasts,HPDLFs)生物学活性的影响。方法:改良乳化冷凝聚合法制备rhIGF-Ⅰ-GMs,ELISA法测定其载药特性;体外培养HPDLFs,rhIGF-Ⅰ和rhIGF-Ⅰ-GMs梯度含量分别作用于HPDLFs,酶动力学方法及ELISA法动态观察其碱性磷酸酶(alka-linephosphatase,ALP)活性及对合成纤维结合蛋白(fibronection,Fn)的影响。结果:微球表面光滑圆整,微球载药量和包封率分别为930ng/g和93.0%;培养1d后,对照组、rhIGF-Ⅰ组和rhIGF-Ⅰ-GMs组人牙周膜成纤维细胞ALP活性和合成Fn均无显著性差异(P>0.05);3d后,各组人牙周膜成纤维细胞ALP活性和Fn的合成依次为rhIGF-Ⅰ-GMs组>rhIGF-Ⅰ组>对照组,rhIGF-Ⅰ-GMs组和rhIGF-Ⅰ组比较,有显著性差异(P<0.01)。结论:rhIGF-Ⅰ-GMs及其冻干粉剂制备良好;rhIGF-Ⅰ-GMs通过对rhIGF-Ⅰ的缓释作用,可较长时间地增强HPDLFs的ALP活性和促进Fn的合成。 Objective： To investigate the bioactivities of controlled release rhIGF-I-loaded gelatin microspheres （rhlGF-I-GMs） and their effects on cultured human periodontal ligament fibroblasts. Methods： The rhIGF- I -loaded gelatin microspheres were prepared by improved emulsified cold-condensation method. The content of rhIGF-I-GMs was measured by ELISA method. HPDLFs were cultured in vitro, ALPase activity was measured by enzyme kineticmethods, and fibronectin （Fn） was measured by ELISA after rhlGF- I -GMs and rhIGF- I being added to the culture medium of I-IPDLFs. Results： The rhlGF- I -GMs were good, even and uniform spheres. The drug loading amount and encapsulation efficiency of rhlGF- I -GMs were 930 ng/g and 93.0%. The in vitro cellular study showed no significant difference in ALPase activity and Fn levels of three groups 1 day after plate culture, but there was a great difference among the three groups after 3 days （P〈0.01）. ALPase activity and synthesis of Fn were rhIGF-I-GMs group〉rhIGF- I group〉 control group in order. Conclusion： The rhIGF-I-GMs and their lyophilized powder have excellent pharmaceutical properties. The rhIGF- I -GMs can significantly promote the ALPase activity and Fn levels of HPDLFs by sustained release rhIGF-I .

作者李玲杨路赵志娟张晓霞

机构地区山西医科大学第一临床医学院口腔科山西医科大学药学院

出处《中国医药导报》 CAS 2009年第29期14-16,共3页 China Medical Herald

关键词重组人胰岛素样生长因子-Ⅰ 缓释人牙周膜成纤维细胞 rhIGF- I Sustained release Human periodontal ligament fibroblasts

分类号 R329.2 [医药卫生—人体解剖和组织胚胎学]

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