鞘内注射GluR6反义寡核苷酸减轻大鼠急性直肠炎性痛(英文)

Intrathecal injection of GluR6 antisense oligodeoxynucleotides alleviates acute inflammatory pain of rectum in rats

目的探讨海藻氨酸受体亚单位GluR6在急性内脏炎性痛中的作用。方法在SD大鼠直肠粘膜下注射福尔马林建立内脏痛模型。鞘内注射GluR6反义寡核苷酸,通过行为学评分观察注射后大鼠疼痛行为学的变化,并用免疫印迹法检测GluR6蛋白的表达变化。结果直肠内注射福尔马林后大鼠脊髓GluR6表达升高。鞘内注射GluR6反义寡核苷酸后,在福尔马林注射后45min时,GluR6蛋白表达降低,大鼠疼痛行为学评分显著降低。结论GluR6在福尔马林致大鼠直肠炎性痛中可能起重要作用,这为临床治疗内脏痛提供了新思路。

Objective To investigate whether the kainate （KA） receptor subunit GluR6 is involved in the acute inflammatory pain. Methods Formalin was injected into the mucosa of rectum in Sprague-Dawley rats to induce visceral pain. The antisense oligodeoxynucleotides （ODNs） of GluR6 were injected once per day for 3 d before formalin injection, after which GluR6 protein level was examined by immunoblotting method. The change of visceral pain was also investigated. Results The expression of GluR6 in the spinal cord of rats increased after the formalin injection. Moreover, pre-treatment of GluR6 antisense ODNs could suppress GluR6 expression in the spinal cord of rats and decrease the scores of visceral pain at 45 min following formalin injection. Conclusion Kainate receptor subunit GluR6 plays an important role in the visceral pain induced by injection of formalin into the wall of rectum. GluR6 may serve as a potential target for the treatment of acute inflammatory visceral pain.