兔VX2肝癌模型的建立及其生长转移特性的观察

The establishment of VX2 hepatic carcinoma model in rabbits and the observation of its growing and metastatic characteristics

目的探讨超声引导下经皮穿刺瘤块推送法建立兔VX2肝癌模型的可行性,评估开展介入治疗实验研究的最佳时期。方法28只新西兰大白兔接受VX2瘤块接种,接种后2、3、4周行超声检查及PET/CT检查,并分别处死2只建模成功的动物行病理检查。结果建模成功率为89.3%(25/28)。接种后2、3、4周肿瘤最大径分别为(4.82±0.80)mm、(16.05±2.89)mm、(30.08±5.38)mm,转移率分别为0(0/25)、13.0%(3/23)、76.2%(16/21);接种后2周肿瘤生长旺盛,无明显坏死,3周仅有少量点片状凝固性坏死,4周见大片坏死。结论超声引导下经皮穿刺瘤块推送法建立兔VX2肝癌模型简单易行,成功率高;宜在接种后第3周开展介入治疗的实验研究。

Objective To establish VX2 hepatic carcinoma model in rabbits by implanting the tumor fragment into the liver through percutaneous puncture under ultrasound guidance and to observe its growing and metastatic characteristics, to determine the optimal time for interventional experiment study with the model. Methods Inoculation of VX2 carcinoma fragment was performed in 28 New Zealand white rabbits. PET/CT and uhrasonography （US） examinations were carried out in the second, third and forth week after the inoculation, and each time two tumor-hearing rabbits were sacrificed for pathologic study. Results The successful rate of model establishment was 89.28% （25/28）. On PET or CT scans, single lesion in the liver was demonstrated in 25 rabbits. Two, three and four weeks after the inoculation, the maximum diameter of the tumor was （4.82 ± 0.80） mm, （16.05 ± 2.89） mm and （30.08 ±5.38） mm respectively, while the metastasis rates was 0% （0/25）, 13.04% （3/23）, 76.19% （16/21） respectively. No significant necrosis was found in the second week after inoculation, only tiny coagulation necrosis was revealed in the third week, and massive necrosis was seen in the forth week. Conclusion Percutaneous inoculation of the tumor fragment into the liver under uhrasonographic guidance is a simple method to establish VX2 hepatic carcinoma in rabbits with a high successful rate. The third week after inoculation is the suitable time for making interventional experiment study.