stathmin基因在喉鳞状细胞癌组织中的表达及意义

The expression stathmin gene in laryngeal squamous cell carcinoma

目的:探讨stathmin基因在人喉鳞状细胞细胞癌(LSCC)的表达及其与LSCC发生、发展之间的关系。方法:原位杂交方法检测stathmin mRNA在35例人LSCC组织及18例癌旁正常喉黏膜组织(NLT)中的表达情况。结果:在35例LSCC组织中有24例stathmin mRNA呈阳性表达(阳性率69%),阳性颗粒可见于细胞质及细胞核,18例NLT中6例呈弱阳性表达,差异有统计学意义(P<0.05)。结论:stathmin基因在LSCC组织中高表达,stathmin基因高表达与LSCC组织的发生、发展有重要的相关性。

Objective：To observe expression of stathmin gene in laryngeal squamous cell carcinoma（LSCC） and relation between expression of stathmin gene and occurance and development of LSCC. Method：The expression of the stathmin gene was determined in 35 LSCC of specimens and 18 normal laryngeal tissues（NLT） of specimens by in situ hybridizaion with Digoxigenin labled probe of stathmin mRNA. Result.. Expression of stathmin gene was observed in 35 cases of laryneal squamous cell carcinoma tissue （positive rate, 69%） and positive signal was observed in both cytoplasm and nuclear. Among 18 cases of normal tissue, only 6 showed weak positive signal. There was significant difference in expression of stathmin gene between laryneal squamous cell carcinoma tissue and nomal tissue. Conclusion：Expreesion of stathmin gene may play a key role in the pathogenisis and development of laryneal squamous cell carcinoma. It may be a very important biotherapy target in the treatment of laryneal squamous cell carcinoma.