直立型与匍匐型川麦冬的综合比较研究

Comprehensive comparison between erective and creepy Ophiopogon japonicus of Sichuan

目的:比较不同株型川麦冬农艺性状、HPLC图谱、大类成分含量、氨基酸含量间的差异,为川麦冬资源评价及质量控制研究提供理论依据。方法:采用常规方法测定麦冬农艺性状;以高效液色谱法,用槲皮素作为内标参照物测定其甲醇提取部位HPLC图谱;以紫外分光光度法测定其总皂苷、总黄酮、可溶性糖含量;以氨基酸自动分析仪测定其氨基酸含量。结果:不同株型川麦冬多数农艺性状间均存在极显著差异,块根数、地上部分鲜重与其单株产量的相关性最大;不同株型川麦冬HPLC图谱69个共有峰及23个非共有峰相对峰面积间的差异均不显著;各大类成分含量间的差异均未达到显著水平。结论:不同株型川麦冬主要化学成分的含量无明显差异,块根数多、地上部分重的川麦冬,其单株产量较高。生产上宜选择块根数多、地上部分重的匍匐型麦冬作为栽培植株。

Objective：To compare the agronomic characters, HPLC fingerprints, the content of main component and amino acid between ereetive and creepy Ophiopogon japonicus of Sichuan. Method： Agronomic characters were measured by conventional methods; HPLC was applied on a C18 column with CH3OH-CH3 CN-2% CH2COOH solution by gradient elution, quercetin was used as the internal standard reference, the contents of total saponins, flavonc and polysaccharide were determined by UV spectrophotometry and amino acid was determined by automatic amino acid analyzer. Result： There were extremely significant differences in the most agronomic characters between erective and creepy O. japonicus of Sichuan. The yield per plant was closely relevant to the roots number and the fresh weight of aerial part. The differences were not significant both in 69 common peaks and 23 uncommon peaks in HLPC fingerprints and the content of main component between ereetive and creepy O. japonicus. Conclusion ： There is no obvious difference in chemistry component between the two types of O. japonicus. The yield per plant was closely relevant to the roots number and the fresh weight of aerial part. In cultivation it is appropriate to choose the creepy O. japonicus.