丹皮炭HPLC指纹图谱研究

HPLC fingerprints of Cortex Moutan Carbonisatum

目的：建立丹皮炭的HPLC指纹图谱分析方法，为整体控制和评价丹皮炭的质量提供依据。方法：采用Hedera ODS-3柱（4．6mm×150mm，5μm），流动相甲醇-乙腈（1：1）-0．5‰三氟乙酸水，梯度洗脱，检测波长258nm。以丹皮酚为参照物，对10批丹皮炭进行指纹图谱的分析。结果：建立了丹皮炭饮片的HPLC指纹图谱共有模式，标定了11个共有峰，10批炭品的相似度在0．830～0．991。结论：该指纹图谱方法简便、准确、重复性好，可专属性地研究丹皮炭的指纹图谱，为有效地控制该饮片的内在质量提供了科学依据。

Objective ： To establish the analytical method of the HPLC fingerprint of Cortex Moutan Carbonisatum and offer the evidence for quality control and quality evaluation of carbonized Cortex Moutan accordingly. Method： Ten batches of Cortex Moutan Carbonisatum were measured by HPLC with Paeonol as a reference substance and the HPLC analysis was performed on a Hedera ODS- 3 Chromatographic Column （4. 6 mm×150 mm, 5 μm）, with methanol to Acetonitrile （ 1：1 ） and 0. 5‰ trifluoroacetic acid solution as mobile phase in gradient mode and the detection wavelength was at 258 nm. Result： The common mode of the HPLC fingerprints were set up. There were 11 common peaks in the fingerprint of ten samples, and the similar degrees to the ten batches were between 0. 830-0. 991. Conclusion： The method is simple,accurate and have a good reaptability. And the quality of Cortex Moutan Carbonisatum can be controlled effectively by the HPLC fingerprint.