HPLC测定人血浆中的10α-甲氧基-6-甲基麦角林-8β-甲醇

Determination of 10α-methoxy-6-methylergoline-8β-methanol in human plasma by HPLC

目的采用HPLC测定人血浆中尼麦角林主要代谢物10α-甲氧基-6-甲基麦角林-8β-甲醇（MDL）的血药浓度。方法色谱柱为Luna C18柱（250 mm×4.6 mm,10μm）,流动相为乙腈-0.01 mol·L^-1磷酸氢二钠（15：85,磷酸调pH3.5）,流速为1.0 ml·min^-1,柱温30℃,检测波长为225 nm。结果线性范围为5.0～80.0 ng·ml^-1（r=0.9997,n=7）,平均方法回收率分别为93.85%、94.83%、89.84%;日内RSD分别为4.93%、8.23%、10.63%（n=5）,日间RSD分别为6.17%、6.13%、8.41%（n=5）。结论该法灵敏、简便、准确,适用于尼麦角林及相关制剂的临床研究。

OBJECTIVE To establish the method for determination of 10α - methoxy - 6 - methylergoline - 8β - methanol （ MDL, a main metabolite of Nicergoline） in human plasma by HPLC. METHODS Chromatographic column was based on Luna C18 （250 mm × 4.6 mm, 10 μm）. The mobile phase consisted of acetonitrile： 0.01 mol·L^-1 disodium hydrogen phosphate （15 ： 85, pH3.5 ）with a flow rate of 1.0 ml·min^-1. The column temperature was 30℃. The detecting wavelength was 225 nm. RESULT The linear range was 5.0 - 80.0 ng·ml^ -1 （ r = 0. 9997, n = 7 ）. The average method recovery were 93.85% ,94.83% and 89.84 %. The intra - day RSD were 4.93 %, 8.23 % and 10.63 % （ n = 5 ）, and the inter - day RSD were 6.17% ,6.13 % and 8.41% （ n = 5 ）. CONCLUSION The method was sensitive, simple, and reliable, and suitable for determination of nicergoline and other correlated dose forms of nicergoline on clinical study.