基于HPLC法的向日葵盘中绿原酸含量测定

Determination of Ahlorogenic Acid in Sunflower Plate Based on High Performance Liquid Chromatography

［目的］采用HPLC法测定向日葵盘绿原酸的含量。［方法］采用高效液相色谱法。其中，色谱柱为Hypersil ODS（4.6 mm×200 mm，5 μm），甲醇-冰醋酸-乙腈（18∶35∶2）为流动相，流速为1.0 ml/min，检测波长为327 nm。［结果］绿原酸在3.24～89.21 μg/ml范围内线性关系良好,回归方程为：C=23.63A-3.98,r=0．999 8，平均回收率为98.12％，RSD=0.27％。［结论］所建方法准确可靠，操作简便，稳定性好，可用于绿原酸生产的质量控制。

[ Objective ] The research aimed to determine ehlorogenic acid content in sunflower plate based on using HPLC method. [ Method ] Chlomgenic acid was determined by HPLC method. Hypersil ODS（4.6 mm× 200mm,5 μm）was used and the mobile phase consisted of methanol-glacial acetic acetic-acetonitrile （18：35：2） ）at a flow rate of 1.0 mL/min. The detection wavelength was set on 327 nm. [ Result ] The linear range of chlorogenic acid was 3.24 - 89.21 μg/ml. The regression equation was C = 23.63A - 3.98, （ r = 0. 999 8 ）. The average recovery was 98.12% ,with RSD of 0.27%. [ Conclusion] The method is simple,accurate and stable,and it can be used for the quality eontrol of suntlower plate.