胶束电动毛细管色谱法测定人血浆中茶碱的浓度

Determination of Theophylline Concentration in Human Plasma by Micellar Electrokinetic Capillary Chromatography

目的:建立以胶束电动毛细管色谱法测定人血浆中茶碱浓度的方法。方法:血浆样品不用任何前处理直接进样测定,采用未涂层石英毛细管(有效长度21cm),其中阴极缓冲液(A液)为15mmol.L-1的硼砂(pH10),运行缓冲液(B液)为内含200mmol.L-1十二烷基硫酸钠(SDS)的A液,压力进样,分离电压为12kV,紫外检测波长为280nm,外标峰面积法定量。结果:平均每个样品测定11min;茶碱血药浓度在1.25～40μg.mL-1范围内线性关系良好,最低检测限为0.5μg.mL-1;平均方法回收率为90.33%(RSD=2.51%);日内、日间RSD分别为2.34%～3.36%、2.03%～6.51%。结论:本方法方便、快捷、灵敏,适用于茶碱的治疗药物监测。

OBJECTIVE： To develop a simple micellar electrokinetic capillary chromatography for determination of theophylline concentration in human plasma. METHODS： The determination of plasma sample（without any pretreatment） was performed on un - coated fused - silica capillaries （at an effective length of 21 cm） in which the cathodic buffer solution （solution A） was 15mmol·L^-1 borax（pH = 10） and the running buffer solution（solution 13） was sodium dodecyl sufate （SDS） （200 mmol·L^-1） - contained solution A. The sample was injected into capillary by pressure with separation voltage of 12 kV; the UV detection wavelength was set at 280 nm; quantitation was performed by external standard peak area method. RESULTS： The average determination time of each sample was 11 min; the linear range of theophylline was 1.25-40 μg·mL^-1 with its lowest detectable limit at 0.5μg·mL^-1; the average methodological recovery was 90.33% （RSD -2.51% ）; the intra-day RSD was 2.34%--3.36% and the inter- day RSD was 2.03%--6.51%, respectively. CONCLUSION： The developed method is simple, rapid and sensitive and it is applicable for the therapeutic drug monitoring of theophylline.