rmtB基因在铜绿假单胞菌烧伤分离株中流行

Detection of 16S rRNA Methylase and Aminoglycoside Modifying Enzymes and qacE△1-sul1 and merA in Pseudomonas aeruginosa in Burn Unit

目的了解分离自烧伤科患者的铜绿假单胞菌中16S rRNA甲基化酶基因、氨基糖苷类修饰酶基因、耐消毒剂-磺胺基因(qacE△1-sul1)、转座子基因(merA)存在状况。方法以K-B法测量铜绿假单胞菌对20种抗菌药物的耐药性;以PCR法检测20株铜绿假单胞菌16SrRNA甲基化酶及氨基糖苷类修饰酶基因;以PCR直接全自动荧光法进行阳性基因测序。结果20株铜绿假单胞菌检出3种氨基糖苷类修饰酶基因,分别为aac(6′)-Ⅰb、aac(6′)-Ⅱ、ant(2″)-Ⅰ,检出1种16S rRNA甲基化酶基因(rmtB);所测20株菌均携带qacEΔ1-sul1(阳性率100.0%),19株携带merA基因(阳性率95.0%)。结论分离自烧伤科患者的铜绿假单胞菌耐药情况严重,对氨基糖苷类抗菌药物耐药与5种氨基糖苷类修饰基因及16S rRNA甲基化酶基因rmtB有关,qacE△1-sul1及merA携带率高,说明转座子及整合子是细菌间基因传播的重要机制,必须寻求新的途径来解决此类细菌对氨基糖苷类抗菌药物的耐药问题。

OBJECTIVE To explore the distribution of 16S rRNA methylase and aminoglycoside modifying enzymes and qacE△1-sull and merA in isolates of Pseudornonas aeruginosa （PAE） in burn unit. METHODS The antibiotics susceptibility was tested by K-B method, the 16S rRNA methylase （rmtB）and aminoglycoside modifying enzymes and qacEil-sull and merA were detected by polymerase chain reaction （PCR）. RESULTS Among 20 strains of PAE, 3 kinds of aminoglycoside modifying enzymes and Ⅰ kind of 16S rRNA methylase had been detected. The 3 kinds of aminoglycoside modifying enzymes were aac （6′）- Ⅰ b, aac （6′）-Ⅱ , and ant （2″）- Ⅰ ,respectively. All of strains harbored qacE△1-sull（positive rate 100%）,19 of 20 strains harbored merA（posirive rate 95%）. CONCLUSIONS Resistance to antibiotics in PAE of our hospital is mainly related to 3 kinds of aminoglycoside modifying enzymes and 16S rRNA methylase enzyme. There are high frequency of qacE△1-sull and merA gene of the PAE in burn unit, which means must cure resistance in PAE to aminoglycosides by new way.