HPLC、LC-MS检测3种亳白芍栽培品种的质量

Quality analysis of 3 species of Radix Paeoniae Alba cultivated in Bozhou by HPLC and LC-MS

目的:建立用HPLC、LC-MS分析亳白芍3种栽培品种质量的方法。方法:采用LC-MS方法对3种栽培品种的化学成分进行分析;HPLC方法测定药材及其加工品中芍药苷、苯甲酸、苯甲酰白芍苷的含量。结果:LC-MS法结合对照品指认出10个特征峰的归属。分析结果表明,不同栽培品种间化学成分差异较大;药材经传统加工后,芍药苷含量明显增加。选出亳白芍栽培品种中4年生"蒲棒"和5年生"线条"为最佳品种。结论:本实验方法简便、快速,结果准确,可为科学评价及有效控制亳白芍不同栽培品种的质量提供依据。

Aim： To establish the HPLC and LC-MC methods for the quality analysis of the different species of Radix Paeoniae Alba cultivated in Bozhou. Methods： The LC-MS method was applied to analyze the chemical composition of the cultivated species, the HPLC method was applied to determine the contents of paeoniforin, ben- zoic acid and paeonivayin in crude drugs and processing products. Results： 10 characteristic peaks were identified by LC-MS with reference standards. The significant difference was observed in the chemical compositions of 3 cultivated species. The significant difference was observed in the chemical compositions of 3 cuhivated spe- cies. The content of paeoniflorin was increased significantly after traditional processing. The results indicated the 4-year-old ＂pubang＂ and 5-year-old ＂xiantiao＂ are the best cultivated species. Conclusion： The methods are simple, rapid and precise to assess and control the quality of different species of Radix Paeoniae Alba cultivated in Bazhou.