大枣缓解甘遂毒性部位致炎作用的效应

Effects of bioactive extracts from Fructus Jujubae in attenuating the inflammation induced by Radix Kansui

目的:对大枣各分离部位拮抗甘遂致炎毒性的效应进行评价,初步确定大枣中具有解毒作用的有效部位。方法:体外培养小鼠脾淋巴细胞和大鼠腹腔巨噬细胞,分别以MTT法分析脾淋巴细胞增殖和Griess法分析大鼠腹腔巨噬细胞NO释放情况。首先观察甘遂各提取部位对脾细胞和巨噬细胞的影响,以确定毒性部位;再以确定的甘遂毒性部位评价大枣不同提取部位对该毒性部位的拮抗作用。在整体动物水平上,采用组织病理切片,观察大枣有效部位对甘遂提取物致小鼠胃肠急性损伤的影响。结果:甘遂致炎的毒性以RK-3(二萜)部位最为明显;大枣提取部位B(小分子糖和氨基酸)、D(黄酮苷)、E(三萜)对甘遂毒性部位RK-3的致炎毒性具有明显的拮抗作用;大枣有效部位对甘遂提取物致小鼠胃肠急性损伤有保护作用。结论:以细胞模型对甘遂致炎的毒性部位进行分析,进而确定了大枣拮抗甘遂致炎毒性部位的活性部位,为进一步进行大枣的减毒活性成分及作用机制研究提供了实验基础。

Aim： To evaluate the effects of bioactive extracts from Fructus Jujubae in attenuating the inflammation induced by Radix Kansui. Methods： Exoteric splenic lymphoeyte（SPL） of mouse and peritoneal maerophage （PMφ） of rat were used as cell model to evaluate the anti-inflammatory effects of fractions from Fructus Jujubae. MTr method was used to assay SPL proliferation, and Griess method was used in NO release of PMφ）. Firstly, Radix Kansui fraction was applied to induce inflammatory effects of the cells, and then, the anti-inflammatory effects of five extracts of Fructus Jujubae were investigated to determine the active fractions in Fructus Jujubae, which probably contribute to the attenuation of Radix Kansui. The effects of Fruetus Jujubae extract in interfering the gastrointestinal acute damages caused by the extract of Radix Kansui were observed according to the pathological sections of mice. Results： The extract RK-3 （diterpenes） exhibited the most significant pro-inflammatory effect. The bioaetive extracts B（ micromolecule saccharides and amino acids）, D（ flavonoid glycosides）, and E （triterpenes） of Fructus Jujubae could attenuate the action of RK-3. The effective extract of Fructus Jujubae protected stomach and duodenum of mouse from acute damages caused by the extract of Radix Kansui. Conclusion： Fraction RK-3（diterpenes） of Radix Kansui had pro-inflammatory effects, and some extracts of Fructus Jujubae could attenuate this effect of Radix Kansui. The results helped to clarify that Fruetus Jujubae attenuates the toxic effect of Radix Kansui in certain cases, and the bioactive extracts of Fruetus Jujubae discovered in the experiments offer the basis for further research to the active components of Fructus Jujubae.