低温恒能量同步荧光法同时快速检测食品中多种多环芳烃

Simultaneous Determination of PAHs in Food by Low-Temperature Constant-Energy Synchronous Fluorescence Spectrometry

恒能量同步荧光法应用于多环芳烃的检测可以提高选择性,低温可使谱带呈指纹特征,提供常温光谱无法获得的光谱细节信息,有助于实现对复杂基体中多环芳烃的检测。文章结合恒能量同步荧光扫描技术与斯波斯基低温技术,建立了食品中多种多环芳烃的低温恒能量同步荧光同时快速分析方法。对低油脂样品直接用正辛烷浸泡,高油脂样品也只需要增加皂化萃取,即可进行光谱扫描来检测食品样中的多种多环芳烃。对两种类型的实际样进行加标回收实验,回收率为80.2%～98.9%,定量工作曲线线性较好(r≥0.9938)。该方法选择性好、操作简便快捷、费用低廉。

The authors developed a rapid analytical method that could determine the PAHs in food samples simultaneously by the coupled approach of Shpol＇ skii spectroscopy and constant-energy synchronous fluorescence scanning technique. Low-fat food samples, which were just extracted with octane and high-fat food samples pre-processed with saponification and extracting, could be analyzed immediately. The recoveries ranged from 80. 2 % to 98. 9 % and the correlation coefficients of the linearity were higher than 0. 993 8. This method was selective, simple, rapid and inexpensive.