Hepa1-6细胞的培养及小鼠皮下移植瘤模型的建立

Murine Subcutaneous Transplantation Model of Hepatoma with Hepa1-6 Cell

目的培养小鼠Hepa1-6细胞,建立小鼠皮下肝癌模型,为后续实验奠定基础。方法Hepa1-6体外培养,观察细胞生长情况,按2×105/鼠(A组)、2×106/鼠(B组)和4×106/鼠(C组)的细胞量,接种于C57BL/6j小鼠,观察肿瘤生长情况。结果细胞生长速度较慢,传代第3 d为指数生长期;成瘤率分别为A组30%,B组为80%,C组为100%;A、B组分别在接种肿瘤细胞后12 d和10 d成瘤,而C组在接种肿瘤细胞后7 d成瘤,成瘤速度明显高于前两组;第3周和第4周C组和B组肿瘤生长体积显著高于A组(P<0.01),而C组和B组之间无明显差异。结论使用Hepa1-6细胞以4×106/鼠的细胞用量在SPF级雌性C57BL/6j小鼠前肢部皮下成功建立了肝癌移植瘤,完成了小鼠肝癌动物模型的建立。

Objective To develop a murine subcutaneous transplantation model of hepatoma with cell line Hepal-6. Methods Hepal-6 cell was cultured in vitro and its growth was monitored; Hepal-6 cells were injected intradermally into C57BL/6J mice at concentrations of 2× 10^5/mice （A group）, 2 × 10^6/mice（B group）and 4× 10^6/mice（C group）. Results The cells grew very slowly, and they went into exponential growth three days after assimilation; the rate of tumorigenesis in group A, B and C was 30%, 80% and 100% respectively; Tumorigenesis was observed after 12 and I0 days in group A and B respectively, but after 7 days in group C; From the third to the forth week, the tumor bulks in group C and B grew significantly bigger than those in group A （P 〈 0.01 ）. There was no obvious difference in tumor bulks between Group C and B. Conclusion The murine hepatoma model can be established by subcutaneous transplantation of Hepal-6 cells at the concentration of 4 × 10^6/mice.