慢性胃炎病变黏膜组织HSP70的表达与幽门螺杆菌感染的相关性研究

Relevance of HSP70 expression to Helicobacter pylori infection in chronic gastritis mucosa lesions

目的探讨导致慢性胃炎的主要病因幽门螺杆菌(Hp)感染与病变黏膜组织中热休克蛋白70(HSP70)表达的相关性,旨在为临床判定慢性胃炎的严重程度、发展趋势和治疗前景提供依据。方法收集慢性胃炎胃黏膜胃镜活检标本71例,所有标本均经病理证实,其中浅表性胃炎38例,萎缩性胃炎33例。经吉姆萨染色结合临床14C尿素呼气试验测定Hp,把所有病例分为Hp阳性组和Hp阴性组;用免疫组织化学技术测定黏膜组织中HSP70,计算两组黏膜组织HSP70表达阳性率,经统计学方法比较其差异显著性。结果不论浅表性胃炎还是萎缩性胃炎,Hp阳性组黏膜组织中HSP70表达阳性率(分别为61.5%和76.0%)显著高于Hp阴性组黏膜组织HSP70表达阳性率(分别为25.0%和37.5%),差异具有显著性(P<0.05)。结论HSP70在各型慢性胃炎黏膜组织均有表达,Hp感染使其表达量明显增强。测定HSP70可反映Hp感染的严重程度和疾病的发展趋势及治疗前景。

Objective To investigate the relationship between Helicobacter pylori （Hp） infection and the,expression of heat shock protein 70 （HSP70） in gastric mucosal lesions in order to determine the clinical severity and the trend of chronic gastritis, and provide the basis for the treatment. Methods Gastric biopsy specimens were collected from 71 eases of chronic gastritis during endoscopy. Pathological examination of the specimens confirmed that of these cases, 38 were superficial gastritis and 33 were atrophic gastritis. The cases were further divided into Hp positive （ Hp ＋ ） group and Hp negative （ Hp - ） group according to the results of Giemsa staining and 14C urea breath. HSP70 expression in mucosal tissue was analyzed by immunohistochemical technique. The positive rates of HSP 70 expression were then compared between Hp ＋ group and Hp - group. Results The positive rate of HSP70 expression in Hp ＋ group （61.5% for superficial gastritis and 76.0% for atrophic gastritis） was significantly higher than that in Hp - group （25.0% for superficial gastritis and 37.5% for atrophic gastrltis） （ P 〈 0.05 ）. Conclusion HSP70 expression can be found in all types of chronic gastritis mucosa lesions. The expression is significantly enhanced in Hp infected cases. Determination of HSP70 may indicate the severity of Hp infection, the trend of the disease and prognosis