小白菊内酯对结肠癌细胞凋亡的影响及其机制研究

Influence of parthenolide on the apoptosis of human colon cancer cells in vitro and its mechanism

目的观察小白菊内酯（PN）对结肠癌HT-29细胞凋亡的影响并分析其可能机制。方法不同浓度PN作用于结肠癌HT-29细胞，以流式细胞仪（FCM）检测细胞凋亡；Western Blot法检测核转录因子（NF）-B亚单位pso、环氧化酶（cox-2）蛋白表达的改变；ELISA法测定前列腺素E2（PGE2）浓度。结果PN能诱导结肠癌细胞的凋亡，且随PN浓度的增加，结肠癌HT-29细胞的凋亡率增加；而NF-κB亚单位p50、COX-2蛋白表达减少，同时PGE2浓度降低。结论PN可诱导结肠癌HT-29细胞凋亡，其机制可能与其能下调NF-κB、COX-2蛋白表达并减少PGE2生成有关。

Objective To investigate the effect of parthenolide（PN） on human colon cancer cells in vitro and its mechanism. Methods Human colon cancer cell line HT-29 was culture, and treated with PN at the concentrations of 10,50,100,200,500,1 000 μmol/L for 48 hours. Then the apoptosis was detected by flow cytometry. The expression of NF-κB 950 and COX-2 proteins were analyzed by Western blot and the concentration of PGE 2 was measured with ELISA kits. Results PN significantly induced cell apoptosis after 48 h in a dose-dependent manner. The expression of NF-κB pS0 and COX-2 proteins were down-regulated by the PN, and the concentrations of PGE2 were also declined. Conclusion PN can induce apoptosis of the human colon cancer cells. And the process may be carried out through the way of ＂NF-κB→COX-2→PGE2 ＂.