环氧合酶-2和血管内皮细胞生长因子与慢性乙型肝炎肝窦毛细血管化的关系

Relationship between cyclooxygenase-2 and vascular endothelial growth factor with liver sinusoidal capillarization of chronic hepatitis B

目的探讨环氧合酶-2（COX-2）和血管内皮细胞生长因子（VEGF）表达与慢性乙型肝炎（CHB）肝窦毛细血管化（LSC）的关系。方法对200例CHB肝样本作COX-2、VEGF免疫组织化学染色，就其表达变化与LSC的关系进行观察。结果LSC占该组病例80％以上，有轻（灶性）、中（片状）、重度（弥漫）表现。电镜示肝窦内皮撕裂，基膜形成，窦内皮细胞出芽形成血管腔，贮脂细胞转化肌成纤维细胞，COX-2、VEGF、Ⅳ型胶原（Co-Ⅳ）及网状、胶原、弹力纤维表达与LSC的轻重程度呈同步关系。结论肝组织COX-2、VEGF表达增强可促进LSC及肝纤维化。

Objective To study the relationship between expression of cyclooxygenase-2 （COX-2） and vascular endothelial growth factor （VEGF） with liver sinusoidal eapillarization （ LSC ） of chronic hepatitis B （CHB）. We studied liver biopsies from 200 patients with CHB COX-2, VEGF immunohistochemical stain were ohserved to accomplish relationship between expression of COX-2, VEGF and LSC. Results LSC occupy above 80% in the group. There were manifestation in mild-LSC （ focal）, middle-LSC （sheet-shape） and severe-LSC （widespread）. Electron microscope shown the laceration in the endothelium of sinuses and formation of basal lamina and budding formarion lumen of blood vessel and fat-storing cell convert myofibroblast. Expression of COX-2, VEGF, Co-Ⅳ and reticulum, collagen and elastic fibers with mild or severe in LSC is manifest locking relate. Conclusion Increased expression of COX-2, VEGF in liver tissue of CHB may facilitate LSC and hepatic fibrosis.