摘要
在对乌骨鸡多肽的蛋白质含量、水解度及多肽的分子量分布研究基础上,通过测定乌骨鸡活性肽对邻苯三酚自氧化产生的超氧阴离子、Fenton反应产生的羟自由基、DPPH自由基的清除率,还原能力及抑制脂质体过氧化,全面考察了乌骨鸡多肽的体外抗氧化能力。结果表明:乌骨鸡多肽能明显降低邻苯三酚自氧化速率,对羟自由基有极强的清除能力,对DPPH自由基清除率随浓度增加而增加,其IC50分别为1.53、0.051.7mg/mL和1.60mg/mL;进一步研究表明,乌骨鸡多肽具有一定的还原力且与浓度呈正比例关系,同时也能抑制脂质体的过氧化反应。
On the basis of content, degree of hydrolysis and peptide molecular weight distribution of blackbone silky fowl peptides, we comprehensively studied the antioxidant activity in vitro of BSF peptides by measuring scavenging rate of BSF peptides on superoxide anion generated by pyrogallol autoxidation, hydroxyl free radical in Fenton reaction and DPPH free radical, and their reducing power and inhabiting peroxidation of liposomes ability. The results showed that： BSF peptides could significantly reduce pyrogallol autoxidation rate, had highly scavenging capacity of hydroxyl free radical, and had dose dependent in scavenging DPPH free radical scavenging. Their IC50 were 1.53 mg/mL, 0.051.7 mg/mL and 1.60 mg/mL respectively. Futher research showed that BSF peptides had certain reducing power increased as concentration increased, they also could inhibit the peroxidation of liposomes.
出处
《食品科技》
CAS
北大核心
2009年第10期200-204,共5页
Food Science and Technology
关键词
乌骨鸡
多肽
清除率
抗氧化活性
black-bone silky fowl
peptides
scavenging rate
antioxidant activity
