摘要
目的:检测白萝卜中是否存在芥子碱硫氰酸盐。方法:确定了以HPLC法检测白萝卜鲜汁中抗氧化剂芥子碱硫氰酸盐的存在。用VP-ODS SHIMADZU型柱子,乙腈(0.08mol/mL)磷酸二氢钾溶液(15∶85)为流动相,检测波长为318nm。柱温25℃,流速1.0mL/min,进样量10μL。结果:证实白萝卜鲜汁中含有芥子碱硫氰酸盐,但其浓度随着时间的推移,呈有规律的逐步衰退,80min后芥子碱硫氰酸盐的色谱峰基本消失。结论:白萝卜组织受到破坏后芥子碱硫氰酸盐很不稳定。所以,生食白萝卜能更好地利用其抗氧化抗衰老功效。
Purpose： Inspect the Raphanus Sativus L. contains sinapine thiocyanate or not. Method： Identify method-HPLC to test the existing of sinapine thiocyanate in fresh juice of Raphanus Sativus L.. Using the bar model of VP-ODS SHIMADZU, using the acetonitrile（0.08 mol/mL）, potassium dihydrogen phosphate（15：85） as mobile phase, getting the wavelength of 318 nm. Temperature： 25℃, flowing speed： 1.0 mL/min and sample： 10μL. Result： Confirming the existing of sinapine thiocyanate in fresh juice of Raphanus Sativus L., however, as the forwarding of time, the concentration declined step by step in orderliness, the peak in chromatography of sinapine thiocyanate almost disappeared after 80 minutes. Conclusion： After the destroy organization of Raphanus Sativus L., sinapine thiocyanate was very instable. As a result, eating the raw Raphanus Sativus L. （radish） can make better use of its anti-aging function.
出处
《食品科技》
CAS
北大核心
2009年第10期259-262,共4页
Food Science and Technology
基金
江西省教育厅科技基金项目(GJJ08311)
