摘要
从湖南、湖北、河北等地区菜地采集土样,采用抑菌圈法筛选对白菜黑斑病病原菌芸薹链格孢菌(Alternaria brassicae)具有拮抗作用的芽孢杆菌菌株。通过初筛得到具有拮抗作用的菌株20株,复筛出1株拮抗活性较高的菌株LBC-1。通过生理生化试验、形态特征观察及16S rDNA序列分析对其进行了种属鉴定。鉴定结果为菌株LBC-1属于解淀粉芽孢杆菌(Bacillus amyloliquefaciens),是1株广谱拮抗芽孢细菌菌株。
Twenty strains of Bacillus were isolated by sampling soil from fields of various regions via preliminary screening. They have antagonistic activities against Alternaria brassicae. A strain named LBC - 1 with a relatively higher antagonistic activity was obtained via secondary screening. Strain LBC - 1 was characterized by physiological and biochemical experiments, morphological features observation, and 16S rDNA sequence analysis. Based on the results, strain LBC - 1 was finally identified as Bacillus amyloliquefaciens. Strain LBC -1 was a broad-spectrum antagonistic strains of spore-forming bacteria.
出处
《中国蔬菜》
北大核心
2009年第20期29-34,共6页
China Vegetables
关键词
拮抗细菌
筛选
鉴定
解淀粉芽孢杆菌
Antagonistic bacteria
Screening
Identification
Bacillus amyloliquefaciens
