用于黄瓜白粉病抗病性鉴定的SCAR标记

SCAR Markers for Identification of Cucumber Powdery Mildew Resistance

将黄瓜白粉病抗性相关基因的一个共显性AFLP标记成功地转换为简单、实用的SCAR标记。根据AFLP标记片段序列信息设计了两对特异性引物SCPM166和SCPM66。PCR扩增结果显示,SCPM166在抗病亲本、抗病单株和杂合类型单株中均扩增出了大小为166 bp的特异片段,感病亲本与感病单株均无此特异片段,表明该SCAR标记可以鉴定纯合感病个体;SCPM66在感病亲本、感病单株和杂合类型单株中均扩增出了大小为66 bp的特异片段,抗病亲本与抗病单株均无此特异片段,表明该SCAR标记可以鉴定纯合抗病个体。若将两个显性SCAR标记结合起来,相当于一个共显性的SCAR标记。

The co-dominant AFLP marker was successfully converted into simple and applied SCAR markers. Two special SCPM166 and SCPM66 were designed according to the sequence information of AFLP fragments. The SCAR -PCR result showed that the resistant parent, resistant F2 plants and hybrid plants possessed 166 bp fragment, while the susceptible parent and susceptible F2 plants did not possess any fragments by primer SCPM166. This indicated that the SCAR marker could identify susceptible homozygous single plant. The susceptible parent, susceptible F2 plants and hybrid plants possessed 66 bp fragment, while the resistant parent and resistant F2 plants did not possess any fragments by primer SCPM66. This indicated that the SCAR marker could identify homozygous resistant single plant. By joint application of these 2 dominant SCAR markers, homozygous resistant plant and homozygous susceptible plant could be identified simultaneously. It is equivalent to a commonly dominant SCAR marker.