胆道感染大肠埃希菌耐药性和基因型分布的研究

Antibiotic Resistance and Genotype of Escherichia coli in Biliary Tract Infection

目的了解胆道感染大肠埃希菌的耐药性及ESBLs基因型分布状况。方法采用纸片扩散法(K-B法)对大肠埃希菌进行抗菌药物的敏感性试验,通过PCR法分析其所产生基因型。结果71株大肠埃希菌共检出产ESBLs菌23株,产酶率为32.4%,对亚胺培南、美罗培南和头孢哌酮/舒巴坦全部敏感,产ESBLs株对其他抗菌药敏感性较低;基因型分析显示在23株产ESBLs菌中有16株产TEM型酶,4株SHV型酶,12株产CTX-M型酶和5株产OXA型酶。结论胆道感染产ESBLs大肠埃希菌耐药严重,TEM和CTX-M型ESBLs多见。

OBJECTIVE To investigate the drug resistance of Escherichia coli in biliary tract infection and genotype of ESBLs. METHODS Antimicrobial susceptibility test was performed using Kirby-Bauer method. The genotypes of ESBLs were detected by PCR. RESULTS The detection rate of ESBLs producing bacteria was 32. 4% （23 strains）, and the drug-sensitivity test indicated that E. coli was sensitive to imipenem, meropenem and cefoperazone/subactam, but these ESBLs producing strains were resistant to other antimicrobial agents. ESBLs were identified as TEM type in 16 strains, SHV type in 4 strains, CTX-M type in 12 strains and OXA type in 5 strains. CONCLUSIONS ESBLs-producing E. coli in biliary tract infections show strous resistance. TEM and CTX-M are the most common ESBLs genotypes.