基于纳米MnO_2的免标记型的电化学适体传感器用于腺苷的测定

Lable-free detection of adenosine based on nano-MnO_2 modified electrochemical aptasensor

以纳米MnO2作为适体固定的构建平台,制备了一种用于腺苷灵敏测定的电化学交流阻抗型生物传感器。[Fe(CN)6]3-/4-作为氧化还原探针监测传感器表面电子传递电阻的变化,表面电子传递电阻的变化值与腺苷的浓度在1.0×10-9～1.0×10-7mol/L范围内有很好的线性关系,最低检测限为8.0×10-10mol/L。传感器显示出高的灵敏度、好的选择性和稳定性。

A simple and highly sensitive electrochemical impedance spectroscopy（EIS） biosensor based on nano- MnO2 as a platform for the immobilization of the aptamer was developed for the determination of adenosine. In the measurement of adenosine, the change in interfacial electron transfer resistance（Ra） of the biosensor using a redox couple of [Fe（CN）6]3-/4- as the probe was monitored. The change of the electron transfer resistance（ΔRet） of the biosensor was linear with the concentration of adenosine in the range from 1.0 nmol/L to 100 nmol/L, with a detection limit of 8.0×10^-10 mol/L. The fabricated sensor was shown to exhibit high sensitivity, desirable selectivity and good stability.