纳米金结合银增强用于质量放大压电免疫传感器的设计

The design of mass amplified piezoelectric immunosensor with gold nanopaticle and silver enhancement

提出了一种基于纳米金结合银增强的质量放大方法以提高压电免疫传感器的灵敏度。通过夹心法将纳米金引入传感器表面后滴加银增强试剂,在纳米金的催化下可使银沉积在传感器上,从而对免疫反应引起的质量变化进行放大。以检测正常人IgG为例,对该方法的可行性作了研究,设计的传感器可在0.3～5μg/mL范围内实现对正常人IgG的定量检测。

A new approach was proposed to improve the sensitivity of piezoelectric immunosensors based on gold nanopaticle and silver enhancement. Gold nanopaticles were introduced onto the surface of immunosensor by sandwiched immunoassay, then the silver-enhancement solution was dropped to the surface of sensor and subsequently silver deposited around the nanoparticles, which amplified the mass change caused by immunoassay. Sensors for detecting normal human IgG were designed with this method, which allowed the quantitative detection of human IgG in the range of 0.3-5μg/mL.