摘要
用循环伏安法在铂金电极上电聚合一层稳定的天青Ⅰ聚合物膜,研究了这层膜在0.1mol/L磷酸缓冲溶液(pH6.5)中的电化学性质。用纳米金溶胶与聚乙烯醇缩丁醛(PVB)构成复合固酶基质,采用溶胶-凝胶法固定葡萄糖氧化酶(GOD)于天青Ⅰ修饰的铂金电极表面,制成了新型葡萄糖生物传感器。实验发现,所制备的传感器具有响应快、灵敏度高、稳定性好,对葡萄糖的线性响应范围为1.2×10-5～7.5×10-3mol/L,检测下限为6.0×10-6mol/L。并具有抗坏血酸、尿酸干扰的特点。
A new approach to construct an amperometric hiosensor was describe. A stable electropolymerized azure films are electropolymerized on the surface of Pt electrod. Their electrochemical quality is studied extensively in 0.1 mol/L phosphate buffer（pH6.5）. Glucose oxidase（GOD）adsorbed to colloidal gold sols combined with polyvinyl butyral（PVB） immobilized onto the surface of electropolymerized azure films to make a glucose biosensor. The experiments proved that the biosensor exhibited fast response, high sentivity, fine stability and linear dependence on the analyte concentration in the range of 1.2 ×10^-5- 7.5 ×10^-3 mol/L,with the limit of 6.0 ×10^-6mol/L. In the meantime the biosensor can effectively eliminate interference from common interferents, such as ascorbic acid and uric acid.
出处
《化学传感器》
CAS
2009年第3期57-60,共4页
Chemical Sensors
基金
遵义医学院硕士启动基金(F-305)
