基于番红花红聚合膜的生物传感器的应用研究

Study of biosensor and application of poly (safranine T) modified glassy carbon electrode

研究了尿酸(UA)在番红花红(SFR)聚合膜修饰的玻碳电极上的电化学行为,发现SFR聚合膜电极对UA的氧化能够起到明显的电催化作用。分别利用循环伏安法(CV)、差分脉冲法(DPV)、计时电流法研究了UA在pH4.4的磷酸缓冲溶液中的线性关系,发现其浓度分别在2.0×10-5～9.0×10-4mol/L(CV),6.0×10-6～1.0×10-4mol/L、2.0×10-4～8.0×10-4mol/L(DPV),4.0×10-6～1.0×10-4mol/L(计时电流法)范围内呈良好的线性关系。该电极用于实际样品的测定,结果满意。

The electrochemical behavior of uric acid （UA） at poly （safranine T） modified electrode was investigated. It was found that the poly （SFR） modified electrode shows an obvious electrocatalytic activity for the oxidation of UA. In phosphate buffer（pH4.4）, the linear relationship between peak current and concentration of UA was studied by the cyclic voltammetry （CV）, differential pulse vohammetry （DPV）, chronoamperometry method and the linear range for determination of UA was 2.0 ×10^-5- 9.0 ×10^-4mol/L（CV）, 6.0 ×10^-4- 1.0×10^-4mol/L, 2.0 ×10^-4- 8.0 ×10^-4 mol/L（DPV）, 4.0 ×10^-6- 1.0×10^-4 mol/L（chronoamperometry）, respectively. The poly（SFR） modified electrode can be applied to the determination of UA in the practical injection samples with satisfactory results.