原位犬肺热缺血损伤的研究

Warm ischemic injury of orthotopic lung graft in dogs

为了观察热缺血时间对供肺肺功能的影响。将１５只杂种犬分为三组，热缺血时间分别是１、２和０小时（无热缺血的对照组）。实验建立模拟左单肺原位移植（即肺门血管暂时性阻断），观测阻断后６小时内犬氧分压、二氧化碳分压、平均肺动脉压、缺血肺湿重／干重比、肺Ｉ型细胞活性度改变。实验结果表明，尽管热缺血１小时组较无热缺血的对照组肺再灌注损伤严重，但模拟移植肺短期内仍具有较好的肺功能，肺组织损伤轻，而热缺血２小时组则肺功能差。

In order to observe the influence of warm ischemia time (WIT) on canine cadaver donor lungs, 15 adult dogs were divided into three groups, group A with WIT for 1 h, group B with WIT for 2 h, and group C without WIT as control group. In the experiment, a model of mimicking left allograft lung transplantation was established and then PaO 2, PaCO 2, average pressure of the lung artery, wet/try weight ratio of ischemic lung, and type II cell viability were recorded within 6 h to evaluate the function of the lung grafts. The results demonstrated that the cadaver donor lungs in group A had more severe reperfusion damage than in the control group, but the function of the lung grafts was still normal. The function of lung grafts in group B was poor and the damage to the lung tissue severe.