持续低流量灌注对兔心肺联合保存的作用

Effect of low flow perfusion on rabbit heart lung preservation

应用Ｋａｎｅｋｏ兔异位心肺移植模型，研究持续低流量灌注技术对兔心肺联合保存的效果。选用健康家兔３２只，随机均分为对照组及实验组，保存期间，实验组心肺经主、肺动脉持续低流量灌注４℃的相应保存液。心肺移植后，连续观察９０分钟，每３０分钟记录供体左室收缩压、舒张末压和右房压以及气道峰压和动脉血氧分压，以判定移植物心肺功能。实验结束时取心肺组织测定丙二醛含量并计算移植物水分获得。结果实验组移植物水份获得低于对照组，心肺功能优于对照组，再灌注时丙二醛含量低于对照组。认为持续低流量灌注技术保存离体心肺效果优于单纯低温静置保存。

Kaneko's rabbit heart lung transplant model was used to investigate the effect of low flow perfusion (LFP) on rabbit heart lung preservation. Thirty two healthy rabbits were randomly divided into control group and experimental group. The donors in the experimental group were continuously perfused via aorta and pulmonary artery at low flow rate with the same perfusates during preservation. After heart lung transplanted, the donors' LVsp, LVedp, RAP and Paw, and PaO 2 were measured every 30 min for 90 min to evaluate the function of heart and lung. After finishing the trial, the contents of MDA in heart and lung were determined and the water weight gain calculated. The results showed that the rabbits in the experimental group had a significantly lower water weight gain and better cardiac and pulmonary function than in the control group. The experimental group produced less levels of MDA in heart and lung than did the control group. It is concluded that LFP is more effective than cold storage on rabbit heart lung preservation.