携氧液对心脏骤停后脑复苏的作用

Efffects of oxygen carrying fluid on brain resuscitation following cardiac arrest (CA)

目的：将携氧液应用于心跳骤停１０分钟实验犬，旨在探索对心跳骤停后脑复苏的作用。方法：１６条成年健康杂种犬以同法诱颤１０分钟后随机分为两组，实验组（ｎ＝８，携氧液５０ｍｌ／ｋｇ静脉滴注）；对照组（ｎ＝８，非携氧液５０ｍｌ／ｋｇ静脉滴注）；除实验因素（携氧液／非携氧液）外其余处理措施相同。评估神经损害指数（ＮＤ）和整体表现评分（ＯＰＣ），记录生命体征恢复情况，观察了中枢神经系统的形态学变化。结果：与对照组相比，复苏时实验组自主循环恢复时间短；心排量恢复较快并稳定；存活率高；ＮＤ低和ＯＰＣ高；中枢神经缺氧性损害较轻。

Objective:CA is a common clinical situation. The survival rate after CA without brain damage is less than 10%. Thus oxygen supply for cerebral tissue is crucial for cerebral resuscitation following CA. Protecting moribund cerebral cells from further damage becomes one of the most important subjects in cardiopulmonary cerebral resuscitation (CPCR).The experiment is to study effects of oxygen supply for cerebral tissue through venous injection of oxygen carrying fluid after CA. Methods:16 adult mongrel dogs following CA induced by ventricular fibrillation (VF) were divided into 2 groups at random;test group (n=8,after 10 min CA treated with oxygen carrying fluid 50 ml/kg iv drop)and control group (n=8,after 10 min CA treared with non oxygen carrying fluid 50 ml/kg iv drop);Apart from different key study factor (oxygen carrying/non oxygen carrying fluid),all animals underwent the same traditional resuscitation procedures. Neurologic deficit (ND) scores and over all performance categories (OPC) were evaluated. Restoration of spontaneous circulation (ROSC) and cardiac output (CO) were analysed. Cerebral tissue were examed under light microscope and electromicroscope. Results:Compared with control group,the ROSC time was shorter (5.1979±3.886 min VS 12.7625±8.5118 min, P <0.05); CO maintained higherlevel(9.9875±3.1537 vs 6.275±1.2437, P <0.05);72 hours mortality was lower (12.5% vs 50%);and pathomorphological changes of cerebral tissue were mild in test group.Conclusion:The present study suggested that the prognosis of CPCR following CA could be improved by the oxygen carrying fluid.