牛蒡种子萌发初期的代谢研究

Study on Metabolism in Arctium lappa L.at the Primary Stage of Germination

目的：探讨牛蒡种子萌发初期的代谢变化。方法：采用电泳方法检测过氧化物酶和蛋白质的变化。结果：随着牛蒡种子的萌发进程，过氧化物酶的谱带不断加深，一些原有谱带缺失，一些新的谱带出现。可溶性蛋白质的变化主要是颜色深浅的变化，萌发９６ｈ后和萌发１２０ｈ后谱带的变化最明显。结论：牛蒡种子的萌发存在着复杂的代谢过程，萌发９６ｈ和１２０ｈ是代谢的关键时期。

Objective:To investigate the metabolic changes of the seeds at the primary stage of germination in Arctium lappa.Method:By means of electrophoresis the changes of peroxidases and soluble proteins were assayed at the primary stage of germination.Results:The color of some original bands was enhanced,some original bands disappeared,and some new bands were detected in peroxidase patterns during the germinating process.The color changes were the main expressiong in soluble protein patterns.The bands of soluble proteins changed most obviously at 96h and 120h after germination.Conclusion:It follows that the metabolic process in the seeds of Arctium lappa at the stage of germination is complex and 96h and 120h after germination are the key times of metabolism.