养血补肾片对阳虚证动物模型卵巢功能的影响

Effect of Yangxue Bushen Tablet on Ovarian Function in Animal Model of Yang Deficiency

目的 :探讨养血补肾片治疗肾虚型功能性闭经、月经稀发的作用机理。方法 :采用氨基导眠能 (下称氨导 ) ,复制雌性家兔阳虚证模型 ,以克罗米芬为阳性对照药 ,将家兔分为 5组 ,Ⅰ组 :单纯氨导 ;Ⅱ组 :氨导加克罗米芬 ;Ⅲ、Ⅳ组 :氨导加养血补肾片高、低剂量组 ;Ⅴ组 :给蒸馏水组。以模型动物体征 ,卵巢、子宫的组织形态学及β -内啡肽 (β -EP)水平等为指标 ,观察性腺轴功能效应。结果 :Ⅱ、Ⅲ、Ⅳ 3组对阳虚证动物具有整体复健作用 ,观察显示卵巢各级卵泡 ,子宫内膜形态、阴道角化上皮脱落细胞的变化均比Ⅰ组、Ⅴ组有显著性差异 (P <0 0 5 )。血浆β-EP含量Ⅰ组为 1 0 6 6ng/L ,显著低于其他各组 (P <0 0 5 ) ,而Ⅱ、Ⅲ、Ⅳ 3组与Ⅴ组相接近 (P >0 0 5 )。结论 :养血补肾片有调节性腺轴的功能 ,尤其对卵巢功能有促进和调节作用 ,从而达到调经目的。

Objective: To study the therapeutic mechanism of Yangxue Bushen tablet (tablet for nourishing blood and tonifying Kidney) on the Kidney deficiency type of functional amenorrhea and infrequent menstruation. Methods: Applied with orimeten, models of Yang deficiency in female rabbits were made, ikaclomine was taken as the control drug. Group Ⅰ was the group of animal model of Yang deficiency; Group Ⅱ was the ikaclomine treatment group; Group Ⅲ was the high dosage treatment group; Group Ⅳ was the low dosage group; Group Ⅴ normal rabbit treated with distilled water. The functional effect of gonadal axis was evaluated by the changes of the signs of animal histomorphology of ovary and uterus, and the level of β endorphin. Results: The animals of Yang deficiency in Group Ⅱ, Ⅲ and Ⅳ recovered as a whole, and the changes of different levels of folliculi, morphosis of endometria and deciduous vaginal epithelial cell in group Ⅱ, Ⅲ and Ⅳ were more obviously than those in group Ⅰ and Ⅴ (P<0 05). The content of β endorphin in blood plasma in group Ⅰ was 106.6pg/ml, which was lower than those in the other groups (P<0 05), and the contents of β endorphin in blood plasma, in group Ⅲ,Ⅳ and Ⅱ were similar to that in group Ⅴ (P<0 05). Conclusion: Yangxue Bushen tablet has the effect on regulating menstruation by regulating the function of gonadal axis, regulating and promoting the ovarian function.